Our Lyme panel kit tests for four different genes that are found
in Borrelia burgdorferi, the most common cause of Lyme disease in the
United States, and 8 common Lyme disease vector born co-infectors and
other organisms including Borrelia recurrentis, Borrelia miyamotoi,
Babesia microti, Babesia divergens, Babesia duncani, Bartonella bacilliformis, Ehrlichia chaffensis, and Anaplasma phagocytophilum.
Positive detection of Lyme co-infectors indicates likely infection
with the Lyme spirochete as well.
Knowledge is Power
A positive PCR result from the DNA ConneXions Lyme test indicates the presence of DNA from B. burgdorferi and/or other co-infectors. A negative result does not prove a patient is not infected with a tick borne infection, rather it indicates the absence of detectable Lyme and/or other tick borne co-infections. A patients ability to fight the disease, stage of infection, and timing of courses of antibiotics are only some of the factors that may affect the detectability of the spirochetes DNA.
More About Microbes
For additional information on the 11 microbes we test for, please click on the following links:
Watch our helpful video about collecting your sample.
For more information, please contact us: 1.888.843.5832
More About Ordering
Our current Lyme Panel reporting
time is between 2-3 weeks.
Thank you for your patience as we strive to meet the needs of all our loyal customers.
If you are currently in possession of a Lyme Kit that contains paperwork that request credit card information and fee of $650, please do not send that paperwork with your sample.
You will need to call us and provide your payment information to process the $650 fee for your sample.
Our telephone number is 1-888-843-5832.
We're Here for You!
Our staff is standing by Monday – Friday 7am to 5pm MST to assist you with your payment and provide you the necessary information required for your sample to be processed once we receive it. We do apologize for any inconvenience this may cause, however, it is necessary for us to be able to provide the most secure, prompt and accurate processing of payment and samples.
DNA ConneXions® will continue to strive to improve and streamline our payment process to make it as simple and convenient for you as possible, so stay tuned for more information.
Thank you for your patronage and we look forward to hearing from you.
What the science tells us...
Lyme disease cases are concentrated in the Northeast and upper Midwest, with 14 states accounting for over 96% of cases reported to the Center for Disease Control (CDC), with about 300,000 people diagnosed each year. (SOURCE)
Borrelia burgdorferi, is the causative agent of Lyme disease (Lyme borreliosis). The long, thin serpentine morphology is the signature feature shared among spirochetes. The spirochetes in the Borrelia burgdorferi sensu lato genospecies group, cycle in nature between tick vectors and vertebrate hosts. (SOURCE)
In the United States, the age distribution of Lyme borreliosis is typically bimodal, with peaks among children 5–15 years of age and adults 45–55 years of age. The incidence of Lyme borreliosis is higher among men than among women in those <60 years of age, but the sex ratio is nearly equal or slightly higher in women in older age groups. In some European countries, such as Slovenia and Germany, the incidence of Lyme borreliosis is higher among adult women (55%) than among men (45%). In the northeastern United States and in most of Europe, the peak months of disease onset are June and July, which is owing to the feeding habits of nymphal ticks. (SOURCE)
If Lyme disease is not treated early in the course of infection, chronic illness may result and a variety of symptoms may develop. These symptoms include fatigue, musculoskeletal pain, arthritis, cardiac disease and neurological involvement with peripheral neuropathy, meningitis, encephalitis, cranial neuritis and cognitive dysfunction. One viewpoint claims that persistent Lyme disease symptoms are related to ongoing spirochetal infection despite antibiotic therapy. The opposing viewpoint claims that persistent Lyme disease symptoms may be due to spirochetal “debris” without active infection. In this pilot study we demonstrated persistent infection despite antibiotic therapy in 12 North American patients with ongoing symptoms of Lyme disease. Cultures were positive in all 12 patients in our study, indicating that the Borrelia spirochetes were replicating and therefore alive. (SOURCE)
Parasites from the genus Babesia are responsible for causing an emerging zoonotic disease called babesiosis. Babesia microti and Babesia divergens are the most frequent etiological agents associated with human babesiosis in North America and Europe, respectively. Transmission occurs mainly through the bite of a Babesia-infected tick and, less commonly, by blood transfusion. (SOURCE)
Persistent infection is a characteristic feature of babesiosis, a worldwide, emerging tick-borne disease caused by members of the genus Babesia. Persistence of Babesia infection in reservoir hosts increases the probability of survival and transmission of these pathogens. Laboratory tools to detect Babesia in red blood cells include microscopic detection using peripheral blood smears, nucleic acid detection (polymerase chain reaction and transcription mediated amplification), antigen detection, and antibody detection. Babesia microti, the major cause of human babesiosis, can asymptomatically infect immunocompetent individuals for up to two years. (SOURCE)
Babesia microti and Borrelia burgdorferi cause two of the most prominent tick-borne diseases in the U.S.A., human babesiosis and Lyme disease, respectively.
Babesia microti is transmitted by species of Ixodes, the same ticks that transmit the Lyme disease-causing spirochete, Borrelia burgdorferi. B. microti can also be transmitted through transfusion of blood products and is the most common transfusion-transmitted infection in the U.S.A.
Ixodes ticks are commonly infected with both B. microti and B. burgdorferi, and are competent vectors for transmitting them together into hosts.
Few studies have examined the effects of coinfections on humans and those have had somewhat contradictory results.
One study linked coinfection with B. microti to a greater number of symptoms of overall disease in patients, while another report indicated that B. burgdorferi infection either did not affect babesiosis symptoms or decreased its severity.
Mouse models of infection that manifest pathological effects similar to those observed in human babesiosis and Lyme disease offer a unique opportunity to thoroughly investigate the effects of coinfection on the host.
Lyme disease has been studied using the susceptible C3H mouse infection model, which can also be used to examine B. microti infection to understand pathological mechanisms of human diseases, both during a single infection and during coinfections.
We observed that high B. microti parasitaemia leads to low haemoglobin levels in infected mice, reflecting the anemia observed in human babesiosis. Similar to humans, B. microti coinfection appears to enhance the severity of Lyme disease-like symptoms in mice.
Coinfected mice have lower peak B. microti parasitaemia compared to mice infected with B. microti alone, which may reflect attenuation of babesiosis symptoms reported in some human coinfections.
These findings suggest that B. burgdorferi coinfection attenuates parasite growth while B. microti presence exacerbates Lyme disease-like symptoms in mice. (SOURCE)
The Gram-negative bacterial genus Bartonella currently comprises roughly fifteen different species, which cause Bartonellosis in humans. It is known to be transmitted by a vector, primarily fleas and also animal bites, scratches, or needle sticks. However, the clinical implications of many of these human infections are poorly understood, and it is possible that some of the species are non-pathogenic, at least in immunocompetent people. B. henselae or “Cat scratch disease” is reported in approximately twenty thousand cases per year in the United States.
The DNA of various Bartonella species can also be amplified by polymerase chain reaction (PCR) in blood, spinal fluid, and tissue; given the cross-reactivity of the Bartonella antibody tests, PCR may be the most reliable and useful test for Bartonella infection. (SOURCE)
Regional prevalences of Borrelia burgdorferi, Borrelia bissettiae, and Bartonella henselae in Ixodes affinis, Ixodes pacificus and Ixodes scapularis in the USA
The objective of this work was to determine the prevalence of Borrelia and Bartonella species in Ixodes spp. ticks collected from 16 USA states. Genus PCR amplification and sequence analysis of Bartonella and Borrelia16SsRNA-23SsRNA intergenic regions were performed on DNA extracted from 929 questing adult ticks (671 Ixodes scapularis, 155 Ixodes affinis, and 103 Ixodes pacificus). Overall, 129/929 (13.9%) Ixodes ticks were PCR positive for Borrelia burgdorferi sensu stricto, 48/929 for B. bissettiaewhereas 23/929 (2.5%) were PCR positive for a Bartonella henselae. Borrelia bissettiae or B. burgdorferi s.s. and B. henselae co-infections were found in I. affinis from North Carolina at a rate of 4.5%; in a single I. scapularisfrom Minnesota, but not in I. pacificus. For both bacterial genera, PCR positive rates were highly variable depending on geographic location and tick species, with Ixodes affinis (n = 155) collected from North Carolina, being the tick species with the highest prevalence’s for both Borrelia spp. (63.2%) and B. henselae (10.3%). Based on the results of this and other published studies, improved understanding of the enzootic cycle, transmission dynamics, and vector competence of Ixodes species (especially I. affinis) for transmission of Borrelia spp. and B. henselaeshould be a public health research priority. (SOURCE)